Butterfly bush mosaic virus

Family: Buddlejaceae Ornamental. Genus: Alfamovirus Family: Bromoviridae. The virus-infected butterfly bush plants exhibit mosaic and leaf narrowing symptoms.

The virus is transmitted by a large number of aphid species in a non-persistent manner, and also by mechanical sap-inoculation.

For more details of AMV, refer to Medicago sativa. Genus: Potyvirus Family: Potyviridae. ChiVMV infection in plants of Buddleja spp. The virus-infected butterfly bush plants show mild chlorosis and mosaic symptoms. No ilarviruses are reported to infect buddleia and only the nepovirus, Strawberry latent ringspot virus , which is restricted mainly to Europe, is reported to infect this species 1,2.

Total RNA was extracted from the three symptomatic plants and used in reverse transcription-polymerase chain reactions RT-PCR to detect ToRSV and TRSV using primer pairs developed in this laboratory, which amplify regions around the amino terminus of the coat protein of the respective viruses.

Repeated testing by double-antibody sandwich enzyme-linked immunosorbent assay DAS-ELISA of leaves from the plant growing in Clemson consistently produced absorbance values at nm in the range of 0. The ranges of values for the noninfected controls noninfected N. The extraction buffer produced absorbance readings in the range of 0.

References : 1 J. Albouy and J. Virus Diseases of Trees and Shrubs. Chapman and Hill, London, Albouy and J. Virus Diseases of Trees and Shrubs. Chapman and Hill, London, Edwards and R. Pages in: Handbook of Viruses Infecting Legumes.