Qiagen dneasy plant handbook

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Write your own review. DNeasy Plant Mini Kit Reviews Description. Very useful kit. Application Area: Gene expression. No phenol: chloroform steps, and quality of DNA mostly comes good.

Patel Institute of Technology Ease of use 5 out of 5 After sales service 4 out of 5 Value for money 5 out of 5. Amazing results. Application Area: Tissue samples. All results are perfect. Its very good and I need it regularly. Application Area: Isolation of DNA for further genotyping and detection of different microbes in the blood of patients. Germantown, Maryland, and Hilden, Germany, November 5, — QIAGEN announced the worldwide launch of the new DNeasy Plant Pro Kit today, a system for extraction of high-quality DNA from varied plant samples with a range of features that give it a clear best in class position, such as further improved yield and effective inhibitor removal.

The new kit includes the innovative Bead-Beating technology for significantly higher sample disruption efficiency. DNeasy Plant Pro Kit significantly outperforms all broadly available alternatives when it comes to increased yield and efficient inhibitor removal, showing no inhibition of a sensitive IC PCR. Plant samples often contain high levels of secondary metabolites like polysaccharides, lipids, terpenes or polyphenols which are difficult to remove.

Additionally, plant samples come in high diversity from young leaves to seeds of very hard matter, which requires a thorough sample disruption for optimal release of DNA. An optional Phenolic Separation Solution step helps provide pure DNA even from samples with high polyphenolic compounds, such as pine or grape leaves by breaking the bonds between DNA and phenolics.

This step prevents DNA loss that would otherwise occur in these samples. The DNeasy Plant Pro Kit is used with a vortexer or high-velocity bead beating, based on sample needs. Vortex methods work with soft leaf tissue.

High-velocity bead beaters, like the PowerLyzer 24 Homogenizer or the TissueLyser II, break down tougher plant material such as roots, seeds, stems or challenging leaf tissues. Samples are first mechanically disrupted and then chemically lysed see flowchart " DNeasy Plant and DNeasy 96 Plant procedures. RNA is removed by RNase digestion during lysis. Cell debris, precipitated proteins and polysaccharides are removed and the sample is homogenized by centrifugation through a QIAshredder spin column.

Buffering conditions are adjusted and the lysate is loaded onto a DNeasy Plant spin column or well plate. During a brief spin, DNA selectively binds to the silica membrane while contaminants pass through. The remaining contaminants and enzyme inhibitors are removed in one or two efficient wash steps. Pure DNA is then eluted in water or low-salt buffer, ready for use. DNeasy Plant Kits provide purification of ready-to-use DNA from plant samples, including plant cells, plant tissues and fungi.

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results. Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction. Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration.

Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

Alcohol precipitation is commonly used for concentrating, desalting, and recovering nucleic acids. Since less alcohol is required for isopropanol precipitation, this is the preferred method for precipitation of DNA from large volumes.

In addition, isopropanol precipitation can be performed at room temperature, which minimizes co-precipitation of salt that interferes with downstream applications.

Tip: Use a buffer with a pH of 7. Often distilled water can have an acidic pH. Alternatively, dry the plant material in the presence of a fold excess of silica gel and seal it in a plastic bag if the material is not dried completely after 12 hours the DNA is likely to degrade. See Mark W. Chase, Harold H. Hills; Taxon, Vol.

To avoid overloading the DNeasy Mini Spin Column do not use more than mg fresh or frozen tissue, or 20 mg dried plant tissue, for each DNA preparation. The filter can only tolerate low centrifugal forces not sufficient to shear genomic DNA. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards.

DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach. EDTA chelates divalent cations which are required for nuclease activity. It may be possible to leave EDTA out of the storage buffer without negative consequences when samples are kept under these conditions, and when repeated freeze-thaw cycles are avoided.

We do recommend however that gDNA be stored in a neutral to a slightly basic buffered solution e. Note that deionized water mostly has an acidic pH. Reorder now! Reorder from your past orders in just one click. Order by Quote. Quote Number. Add quote number from your quote document. Customer Number.